The sexually dimorphic behaviour of adult Drosophila suzukii: elevated female locomotor activity and loss of siesta is a post-mating response

The polyphagous Drosophila suzukii is a highly invasive species that causes extensive damage to a wide range of berry and stone fruit crops. A better understanding of its biology and especially its behaviour will aid the development of new control strategies. We investigated the locomotor behaviour of D. suzukii in a semi-natural environment resembling a typical summer in northern England and show that adult female D. suzukii are at least 4-fold more active during daylight hours than adult males. This result was reproduced in several laboratory environments and was shown to be a robust feature of mated, but not virgin, female flies. Both males and virgin females kept on a 12 h light:12 h dark (12LD) cycle and constant temperature displayed night-time inactivity (sleep) followed by weak activity in the morning, an afternoon period of quiescence (siesta) and then a prominent evening peak of activity. Both the siesta and the sharp evening peak at lights off were severely reduced in females after mating. Flies of either sex entrained in 12LD displayed a circadian pattern of activity in constant darkness confirming the importance of an endogenous clock in regulating adult activity. This response of females to mating is similar to that elicited in female Drosophila melanogaster by the male sex peptide (SP). We used mass spectrometry to identify a molecular ion (m/z, 5145) corresponding to the poly-hydroxylated SP of D. suzukii and to show that this molecule is transferred to the female reproductive tract during copulation. We propose that the siesta experienced by male and virgin female D. suzukii is an adaptation to avoid unnecessary exposure to the afternoon sun, but that mated females faced with the challenge of obtaining resources for egg production and finding oviposition sites take greater risks, and we suggest that the change in female behaviour is induced by the male SP

Real-Time Big Data: the JUNIPER Approach

REACTION 2014. 3rd International Workshop on Real-time and Distributed Computing in Emerging Applications. Rome, Italy. December 2nd, 2014.Cloud computing offers the possibility for Cyber-Physical Systems (CPS) to offload computation and utilise large stored data sets in order to increase the overall system utility. However, for cloud platforms and applications to be effective for CPS, they need to exhibit real-time behaviour so that some level of performance can be guaranteed to the CPS. This paper considers the infrastructure developed by the EU JUNIPER project for enabling real-time big data systems to be built so that appropriate guarantees can be given to the CPS components. The technologies developed include a real-time Java programming approach, hardware acceleration to provide performance, and operating system resource manage-ment (time and disk) based upon resource reservation in order to enhance timeliness.This work is partially funded by the European Union’s Seventh Framework Programme under grant agreement FP7-ICT-611731Publicad

Improving the predictability of distributed stream processors

Next generation real-time applications demand big-data infrastructures to process huge and continuous data volumes under complex computational constraints. This type of application raises new issues on current big-data processing infrastructures. The first issue to be considered is that most of current infrastructures for big-data processing were defined for general purpose applications. Thus, they set aside real-time performance, which is in some cases an implicit requirement. A second important limitation is the lack of clear computational models that could be supported by current big-data frameworks. In an effort to reduce this gap, this article contributes along several lines. First, it provides a set of improvements to a computational model called distributed stream processing in order to formalize it as a real-time infrastructure. Second, it proposes some extensions to Storm, one of the most popular stream processors. These extensions are designed to gain an extra control over the resources used by the application in order to improve its predictability. Lastly, the article presents some empirical evidences on the performance that can be expected from this type of infrastructure.This work has been partially supported by HERMES (Healthy and Efficient Routes in Massive open-data basEd Smart cities). It has been also partially financed by Distributed Java Infrastructure for Real-Time Big Data (CAS14/00118). It has been also partially funded by eMadrid (S2013/ICE-2715) and by European Union’s 7th Framework Programme ​under Grant Agreement FP7-IC6-318763

An Integrated Framework for Multiprocessor, Multimoded Real-Time Applications

The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-642-30598-6_2In this paper we propose an approach for building real-time systems under a combination of requirements: specification and handling of operating modes and mode changes; implementation on top of a multiprocessor platform; integration of both aspects within a common framework; and connection with schedulability analysis procedures. The proposed approach uses finite state machines to describe operating modes and transitions, and a framework of real-time utilities that implements the required behaviour in Ada 2012. Automatic code generation plays an important role: the system is derived from the functional and timing specification, and implemented according to the abstractions provided by the framework. Response time analysis enables assessing the schedulability of the different operating modes and the transitions between modes.This work was partially supported by the Vicerrectorado de Investigación of the UPV (PAID-06-10-2397), Ministerio de Ciencia e Innovación (TIN2011-28567-C03- 03) and European Union (FP7-ICT-287702)Sáez Barona, S.; Real Sáez, JV.; Crespo, A. (2012). An Integrated Framework for Multiprocessor, Multimoded Real-Time Applications. En Reliable Software Technologies – Ada-Europe 2012. Springer. 18-34. https://doi.org/10.1007/978-3-642-30598-6_21834Wellings, A.J., Burns, A.: A Framework for Real-Time Utilities for Ada 2005. Ada Letters XXVII(2) (August 2007)Real, J., Crespo, A.: Incorporating Operating Modes to an Ada Real-Time Framework. Ada Letters 30(1) (April 2010)Sáez, S., Terrasa, S., Crespo, A.: A Real-Time Framework for Multiprocessor Platforms Using Ada 2012. In: Romanovsky, A., Vardanega, T. (eds.) Ada-Europe 2011. LNCS, vol. 6652, pp. 46–60. Springer, Heidelberg (2011)Joseph, M., Pandya, P.: Finding response times in a real-time system. British Computer Society Computer Journal 29(5), 390–395 (1986)Audsley, N., Burns, A., Richardson, M., Tindell, K., Wellings, A.J.: Applying new scheduling theory to static priority pre-emptive scheduling. Software Engineering Journal 8(5), 284–292 (1993)Real, J., Crespo, A.: Mode Change Protocols for Real-Time Systems: A Survey and a new Proposal. Real-Time Systems 26(2), 161–197 (2004)Harel, D.: Statecharts: A visual formalism for complex systems. The Science of Computer Programming 8(3), 231–274 (1987)Object Management Group: Unified Modeling Language (OMG UML) V2.4 (August 2011), http://www.omg.org/spec/UML/2.4.1Sáez, S., Terrasa, S., Lorente, V., Crespo, A.: Implementing Reactive Systems with UML State Machines and Ada 2005. In: Kordon, F., Kermarrec, Y. (eds.) Ada-Europe 2009. LNCS, vol. 5570, pp. 149–163. Springer, Heidelberg (2009)Burns, A., Wellings, A.J.: Dispatching Domains for Multiprocessor Platforms and their Representation in Ada. In: Real, J., Vardanega, T. (eds.) Ada-Europe 2010. LNCS, vol. 6106, pp. 41–53. Springer, Heidelberg (2010)Barnett, J.: State Chart XML (SCXML): State Machine Notation for Control Abstraction (May 2008), http://www.w3.org/TR/scxml

Addressing resource contention and timing predictability for multi-core architectures with shared memory interconnects

Multi-core architectures are increasingly being used in real-time embedded systems. In general, such systems have more processors than the shared memory modules, potentially causing severe interference over memory accesses. This resource contention could lead to substantial variation on memory access latencies, and thus wide fluctuation in the overall system performance, which is highly undesirable especially for the time-critical applications. In this paper, we address resource contention and timing predictability for multi-core architectures with distributed memory interconnects. We focus on the locally arbitrated interconnect constructed by pipelined multiplexing stages with local arbitration, while the globally arbitrated interconnect employing global scheduling to the same architecture potentially suffers synchronisation issue and requires strict coordination. Our contributions are mainly threefold: (i) We analyse the resource contention across the memory access data path, and report the accurate calculational method to bound the worst-case behaviour. (ii) We compare the average-case behaviour of the locally arbitrated and the globally arbitrated architectures with experiments, demonstrating varying memory latencies caused by the resource sharing issue. (iii) We propose an architectural modification to smooth resource sharing. Evaluations on simulators and FPGA implementations with synthetic memory workload show that the latency variation is significantly reduced, contributing towards timing predictability of multi-core systems

An architecture for reliable distributed computer-controlled systems

In Distributed Computer-Controlled Systems (DCCS), both real-time and reliability requirements are of major concern. Architectures for DCCS must be designed considering the integration of processing nodes and the underlying communication infrastructure. Such integration must be provided by appropriate software support services. In this paper, an architecture for DCCS is presented, its structure is outlined, and the services provided by the support software are presented. These are considered in order to guarantee the real-time and reliability requirements placed by current and future systems

A distributed stream library for Java 8

Java 8 has introduced new capabilities such as lambda expressions and streams which simplify data-parallel computing. However, as a base language for Big Data systems, it still lacks a number of important capabilities such as processing very large datasets and distributing the computation over multiple machines. This paper gives an overview of the Java 8 Streams API and proposes extensions to allow its use in Big Data systems. It also shows how the API can be used to implement a range of standard Big Data paradigms. Finally, it compares performance with that of Hadoop and Spark. Despite being a proof-of-concept implementation, results indicate that it is a lightweight and efficient framework, comparable in performance to Hadoop and Spark, and is up to 5 times faster for the largest input sizes tested

Dynamic and static task allocation for hard real-time video stream decoding on NoCs

Hard real-time (HRT) video systems require admission control decisions that rely on two factors. Firstly, schedulability analysis of the data-dependent, communicating tasks within the application need to be carried out in order to guarantee timing and predictability. Secondly, the allocation of the tasks to multi-core processing elements would generate different results in the schedulability analysis. Due to the conservative nature of the state-of-the-art schedulability analysis of tasks and message lows, and the unpredictability in the application, the system resources are often under-utilised. In this paper we propose two blocking-aware dynamic task allocation techniques that exploit application and platform characteristics, in order to increase the number of simultaneous, fully schedulable, video streams handled by the system. A novel, worst-case response time aware, search-based, static hard real-time task mapper is introduced to act as an upper-baseline to the proposed techniques. Further evaluations are carried out against existing heuristic-based dynamic mappers. Improvements to the admission rates and the system utilisation under a range of different workloads and platform sizes are explored

Peptidergic control of the crop of the cabbage root fly, Delia radicum (L.) Diptera: Anthomyiidae): a role for myosuppressin.

There is much interest in targeting neuropeptide signaling for the development of new and environmentally friendly insect control chemicals. In this study we have focused attention on the peptidergic control of the adult crop of Delia radicum (cabbage root fly), an important pest of brassicas in European agriculture. The dipteran crop is a muscular organ formed from the foregut of the digestive tract and plays a vital role in the processing of food in adult flies. We have shown using direct tissue profiling by MALDI-TOF mass spectrometry that the decapeptide myosuppressin (TDVDHVFLRFamide) is present in the crop nerve bundle and that application of this peptide to the crop potently inhibits the spontaneous contractions of the muscular lobes with an IC50 of 4.4 × 10−8 M. The delivery of myosuppressin either by oral administration or by injection had no significant detrimental effect on the adult fly. This failure to elicit a response is possibly due to the susceptibility of the peptide to degradative peptidases that cleave the parent peptide to inactive fragments. Indeed, we show that the crop of D. radicum is a source of neuropeptide-degrading endo- and amino-peptidases. In contrast, feeding benzethonium chloride, a non-peptide agonist of myosuppressin, reduced feeding rate and increased the rate of mortality of adult D. radicum. Current results are indicative of a key role for myosuppressin in the regulation of crop physiology and the results achieved during this project provide the basis for subsequent studies aimed at developing insecticidal molecules targeting the peptidergic control of feeding and food digestion in this pest species

Peptidergic control in a fruit crop pest: The spotted-wing drosophila, Drosophila suzukii

Neuropeptides play an important role in the regulation of feeding in insects and offer potential targets for the development of new chemicals to control insect pests. A pest that has attracted much recent attention is the highly invasive Drosophila suzukii, a polyphagous pest that can cause serious economic damage to soft fruits. Previously we showed by mass spectrometry the presence of the neuropeptide myosuppressin (TDVDHVFLRFamide) in the nerve bundle suggesting that this peptide is involved in regulating the function of the crop, which in adult dipteran insects has important roles in the processing of food, the storage of carbohydrates and the movement of food into the midgut for digestion. In the present study antibodies that recognise the C-terminal RFamide epitope of myosuppressin stain axons in the crop nerve bundle and reveal peptidergic fibres covering the surface of the crop. We also show using an in vitro bioassay that the neuropeptide is a potent inhibitor (EC50 of 2.3 nM) of crop contractions and that this inhibition is mimicked by the non-peptide myosuppressin agonist, benzethonium chloride (Bztc). Myosuppressin also inhibited the peristaltic contractions of the adult midgut, but was a much weaker agonist (EC50 = 5.7 μM). The oral administration of Bztc (5 mM) in a sucrose diet to adult female D. suzukii over 4 hours resulted in less feeding and longer exposure to dietary Bztc led to early mortality. We therefore suggest that myosuppressin and its cognate receptors are potential targets for disrupting feeding behaviour of adult D. suzukii